American Society of Microbiology ABMM : ABMM

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American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of recurrent fungal skin infections presents with a scaly, erythematous rash on their feet. Microscopic examination of skin scrapings treated with potassium hydroxide (KOH) reveals septate hyphae and arthroconidia. The MOST likely genus of the dermatophyte is:

A) Aspergillus
B) Candida
C) Malassezia
D) Trichophyton

2. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Entamoeba histolytica
B) Cyclospora cayetanensis
C) Cryptosporidium parvum
D) Giardia lamblia

3. A clinical microbiology laboratory is using CRISPR-based technology for rapid and sensitive detection of specific bacterial DNA sequences directly from patient samples. This method relies on the ability of the Cas enzyme to:

A) Detect the presence of specific antibodies produced in response to bacterial infection.
B) Reverse transcribe RNA into complementary DNA.
C) Specifically bind to and cleave target DNA sequences guided by a short RNA molecule.
D) Amplify target DNA sequences using specific primers and thermal cycling.

4. A microbiology laboratory receives a sample of cerebrospinal fluid from a patient with suspected bacterial meningitis. The Gram stain shows Gram-positive diplococci. Culture on blood agar shows alpha-hemolytic colonies that are bile soluble and optochin-sensitive. The MOST likely organism is:

A) Listeria monocytogenes
B) Streptococcus pneumoniae
C) Enterococcus faecalis
D) Streptococcus agalactiae

5. A research laboratory is investigating the mechanism of action of a novel antiviral compound. In vitro studies demonstrate that the compound effectively inhibits the replication of a specific enveloped RNA virus. Further analysis reveals that the compound does not interfere with viral entry, genome replication, or protein synthesis. However, electron microscopy of infected cells treated with the compound shows an accumulation of viral glycoproteins within the endoplasmic reticulum and Golgi apparatus, with a significant reduction in the release of infectious virions. The MOST likely target of this antiviral compound is:

A) Host cell membrane fusion machinery
B) Viral protease responsible for polyprotein cleavage
C) Viral glycoprotein trafficking and maturation
D) Viral RNA-dependent RNA polymerase

Solutions: